WebAfter a 30 minute wash, both lanes were further probed with purified anti-rabbit IgG (clone 6B9G9) antibody. Proteins were visualized using an HRP goat anti-mouse IgG antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control. WebPolyclonal primary antibodies are generally raised in rabbit, goat, sheep or donkey and are generally IgG isotypes. The secondary antibody therefore, will typically be an anti-IgG H&L (Heavy & Light chains) antibody. Monoclonal primary antibodies are commonly raised in mouse, rabbit and rat.
Rabbit anti-Mouse IgG (H+L), HRP (31450) - Thermo …
WebThe Anti-Mouse IgG (H+L), HRP Conjugate, antibody binds to both heavy and light chains for all mouse IgG subclasses. As with all antibodies, in certain applications some species-dependent antigen-dependent cross-reactivity may be observed. The product (unless otherwise noted) is supplied as a 1mg/ml solution. WebGoat Anti-Rabbit HRP (IgG H&L) (ab97051) was used as the secondary antibody at 1/2000 dilution (lanes 1and 2), 1/10000 dilution (lanes 3 and 4) and 1/20000 dilution (lanes 5 and 6). The blot was developed using the ECL technique and performed under reducing conditions. Exposure time was 10 seconds. Anti-mouse HRP secondary antibodies how many months are 88 days
Anti-Mouse IgG (H+L), HRP Conjugate - Promega
WebJan 4, 2024 · mouse monoclonal secondary antibody conjugated to Horseradish Peroxidase (HRP) supplied at 200 µg in 0.5 ml volume Strongly recommended alternative to … WebDesigned for use with rabbit polyclonal and monoclonal antibodies, this affinity purified goat anti-rabbit IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase … WebAnti-rabbit IgG, HRP-linked Antibody#7074 1 ml $182.00 PREVIEW β-Actin (8H10D10) Mouse mAb#3700 100 µl $313.00 PREVIEW Anti-rat IgG, HRP-linked Antibody#7077 1 ml $182.00 GAPDH (D16H11) XP® #5174 $352.00 β-Actin (13E5) Rabbit mAb#4970 $313.00 GAPDH (14C10) Rabbit mAb PREVIEW Product Information Product Description how bad does a scorpion sting hurt