WebTransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase. • TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase. • PCR products can be directly cloned into … WebPhusion ® High-Fidelity DNA Polymerase For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific ® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. 50X higher fidelity than Taq Robust reactions - maximal success with minimal optimization
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WebApr 10, 2024 · PCR was performed in triplicate in a 20 μL mixture containing 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase, and 10 ng of template DNA. WebBrand : TransGen Biotech. Manual. Product Details. TransStart® FastPfu PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu DNA polymerase, dNTPs, and optimized …
Web· Select high-fidelity DNA polymerases: Family B DNA polymerase, such as Pfu DNA polymerase, with 3′→ 5′exonuclease activity, corrects mismatched base pairs to ensure high fidelity. In addition, polymerase blends containing Family B DNA polymerases also have a high fidelity, which is lower than the fidelity of Family B DNA polymerase. WebSep 18, 2024 · The formal PCR test used TransGen AP221-02: TransStart Fastpfu DNA Polymerase, 20 μl reaction system: 5×FastPfu buffer 4 μl, 2.5 mM dNTPs 2 μl, forward primer (5 μM) 0.8 μl, reverse primer (5 μM) 0.8 μl, FastPfu polymerase 0.4 μl, BSA 0.2 μl, template DNA 10 ng, and supplement ddH 2 O to 20 μl. The following thermal cycling …
Web侯 磊，薛 蓓，薛会英 (西藏农牧学院，西藏 林芝 860000) 土壤线虫作为土壤动物中的优势类群，是草地生态系统中的消费者，关系到草地生产力的增减[1]。 WebThe PCR mixtures contain 5 × TransStart FastPfu buffer 4 μL, 2.5 mM dNTPs 2 μL , forward primer (5 μM) 0.8 μL, reverse primer (5 μM) 0.8 μL, TransStart FastPfu DNA …
WebPfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in …
WebApr 11, 2024 · PCR reactions were performed in quadruplicate using a 20 μL mixture consisting of 4 μL of 5× TransStart FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of Forward Primer and Reverse Primer (5 μM), 0.4 μL of FastPfu Polymerase, 0.2 μL BSA, 10 ng Template DNA, and supplemented with sterile water to 20 μL. The qPCR product was … ntp 時刻同期 コマンド linuxWebJul 22, 2024 · PCR amplification was performed using the TransStart ® FastPfu Polymerase system (Transgen Biotech, Beijing, China), including 4 μL 5 × FastPfu Buffer, 2 μL dNTPs (2.5 mM), 0.8 μL each primer (5 μM), 0.4 μL FastPfu Polymerase, and 10 ng template DNA. The reaction mixture was initially denatured at 95°C for 2 min, followed by … agricase toledoWebApr 12, 2024 · FastPfu DNA polymerase and 2× FastPfu reaction buffer (Tolo Biotech., China) ( see Note 3 ). 4. 10 mM dNTP working solution. 5. 0.2 mL thin-walled PCR tubes. 6. 1.5 mL polypropylene microcentrifuge tubes. 7. Thermocycler with heated lid ( see Note 4 ). 8. PCR Clean-Up System. 9. Distilled Water, DNase, RNase-free ( see Note 5 ). 10. agricat solomiacWebTransgen Ap221 02 Transstart Fastpfu Dna Polymerase, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. … ntp 時刻同期 タイミング linuxWebPfu Polymerase is a thermostable DNA polymerase isolated from the archaeal thermophile Pyrococcus furiosus. The 90-kDa Pfu polymerase enzyme features a 3’-5’ exonuclease proofreading ability, allowing for higher fidelity than Taq. The error rate of Pfu has been reported to be at least 10-fold lower than that of Taq. ntp時計 ミリ秒WebJul 15, 2024 · Planktonic eukaryotes are important components of aquatic ecosystems, and analyses of the whole eukaryotic planktonic community composition and function have far-reaching significance for water resource management. We aimed to understand the spatiotemporal variation and drivers of eukaryotic plankton distribution in the Middle … ntp名古屋トヨペット 社長WebApr 14, 2024 · The polymerase in the PCR process for bacterial/fungal DNA was TransStart Fastpfu DNA Polymerase/TaKaRa rTaq DNA polymerase. Twenty-microliter bacteria/fungi mixtures for PCR contained 4 μL of 5× TransStart FastPfu buffer/2 μL of 10× Buffer, 2.5 mM dNTPs in 2 μL, 0.8 μL of forward primer (5 μM), 0.8 μL of reverse primer (5 μM), 0.4 μL ... ntp 名古屋 トヨペット